Medical Laboratory Scientist (MLS) ASCP Practice Exam

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Sodium nitroprusside reacts with which substance on a urine reagent strip?

Glucose

Acetoacetic acid (ketones)

Sodium nitroprusside is specifically used in urine reagent strips to detect the presence of acetoacetic acid, which is one of the ketone bodies. When sodium nitroprusside comes into contact with acetoacetic acid in urine, it reacts to form a color change that can be visually interpreted or measured instrumentally. This reaction is crucial for identifying ketonuria, which can occur in conditions such as diabetic ketoacidosis, starvation, or other metabolic disorders where ketones are produced in excess.

The other substances listed do not react with sodium nitroprusside in the same way. For instance, glucose would typically be tested using a different reagent that specifically identifies reducing sugars, while bilirubin detection relies on a reaction with a diazo reagent, and protein detection usually involves the azo-coupling reaction. Each of these substances has distinct methods for detection on the urine reagent strip that do not involve sodium nitroprusside.

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Bilirubin

Protein

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